University Laboratory Testing

May 2000 - Our Peaceful Mountain Sinus Spray product is undergoing MIC (Minimum Inhibitory Concentrations) testing in a University Research Facility.  First round screening against a wide-spread bacterium which is responsible for respiratory ailments showed very positive results.  The concentrate and first dilutions showed better than 99% inhibition of bacterial growth.  The testing will continue with MBC screening and we will report the results as they become available. 

The DermaSeptic system was evaluated for it's ability to retard and kill Staph. Aureus bacteria in tests performed by an independent laboratory specializing in state-of-the-art invitro models.  After a 48 hour challenge in a slime-producing bacteria environment, the DermaSeptic system killed 70% of the clinging bacteria with a single application. 

Testing of the continuous low-level application of the DermaSeptic to the region in challenge showed no (zero) live bacteria in plate culture tests. This was after a continuous 48 hour challenge.  The control showed colonies "TNTC" or too numerous to count.  After a 10 to 1 dilution, the control still showed greater than 300 CFUs (colony forming units per milliliter). 

Excerpts From Journal Articles Regarding Silver

Some Specific Cellular Effects of Electrically Injected Silver and Gold Ions
J.A. Spadaro and R.O. Becker, Veterans Administration Hospital, Department of Orthopedic Surgery, State University of New York, Upstate Medical Center, Syracuse, New York, 13210 (USA), Presented at the 3rd International Symposium on Bioelectrochemistry, Julich, 27-31 October 1975, Printed in Bioelectrochemistry and Bioenergetics 3, 49-57 (1976)

" Even Antibiotic resistant strains are quite susceptible to the injected silver."

"The antibacterial effects of the silver compounds have been known for a long time. At present, a few silver complexes and silver nitrate are used against local infections. Others have shown that the responsible agent in these compounds is the silver ion,"

"The minimum inhibitory concentrations (MIC) of electrically generated silver ions have been determined for a number of bacterial species in vitro and are generally found to be 1-100 times lower than those for other common antibiotics, including silver sulfadiazine."

"To test for any adverse effects of anodically generated silver on normal mammalian cells, a medium with 4 ug/cm³ of Ag was used to culture mouse bone marrow cells. After 18hrs in culture differential counts were made of this mixed cell population. No abnormal cell lysis, adhesion or distortion was observed. Relative cell populations were found only slightly different from control cultures (without silver). Thus it appears that electrochemically generated silver ions are quite effective against many bacteria, while leaving mammalian cells intact."

Inhibitory and Cidal Antimicrobial Actions of Electrically Generated Silver Ions
Richard E. Hall, DDS, MS, Ph.D., Gary Bender, and Robert E. Marquis, Ph.D. Journal of Oral Maxillofac. Surg., 45, 779, 1987

"Data revealed that various silver salts were nearly equally effective as growth inhibitors based on silver content. However, it was found that LIDC Ag was a more potent growth inhibitor than silver salts at any particular silver level."

Inhibitory Effects of Electrically Activated Silver Material on Cutaneous Wound Bacteria
Alfred E. Falcone, DDS, MD, and Joseph A. Spadaro, Ph.D., Journal of Plastic and Reconstructive Surgery, March 1986, 77,455

"Our studies confirm previous reports demonstrating an effective penetration of the silver ion and its local antibacterial action."

"The silver cation has broad-spectrum activity toward gram-positive, gram-negative, aerobic and anaerobic micro-organisms."

Antifungal Properties of Electrically Generated Metallic Ions
T.J. Berger, J.A. Spadaro, Richard Bierman, S.E. Chapin, and R.O. Becker, Antimicrobial Agents and Chemotherapy, Nov. 1976, p.856-860 American Society for Microbiology Vol.10, No.5

"Growth in all isolates was inhibited by concentrations of electrically generated silver ions between 0.5 and 4.7 ug/ml, and silver exhibited fungicidal properties at concentrations as low as 1.9ug/ml. The inhibitory and fungicidal concentrations of electrically generated silver ions are lower than those reported for other silver compounds."

"All organisms were inhibited by silver concentrations of 4.7ug/ml or less, and the fungicidal concentration of silver was as low as 1.9 ug/ml. These values were generally lower than the values reported for other silver compounds against Candida."

" Golubovich completely interrupted the growth of C. utilis (10⁶ cells/ml) 4.7 ug of AgNO₃ per ml. Our findings show that electrically produced silver not only stops growth at concentrations below that used by Golubovich but also kills some species of Candida in the same range and without nitrate."

"Our results with yeasts show that their MIC and some MFC values are in the same range as those reported for 16 bacterial species. Thus, electrically released silver-ion therapy seems to be just as effective against some unicellular eukaryotic organisms as with prokaryotic ones."

"The data show that electrically generated silver cations are more effective than silver sulfadiazines or silver nitrate."